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  • The (CAG)n repeat polymorphism in exon 1 of the gene encoding AR was amplified using PCR in 20 μl reaction volume with 250 nmol/L primers: forward:5´ GCGCGAAGTGATCCAGAAC 3´ tagged with 6–carboxyfluoresce in and reverse 5´ CTCATCCAGGACCAGGTAGC 3´, 1× Taq buffer (Fermentas, Vilnius, Lithuania) and 1U of Taq DNA polymerase (Fermentas, Vilnius, Lithuania).
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