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  • To study MSCs differentiation during cartilaginous TE substitutes production, BMMSCs were trypsinized at the end of the predifferentiation at passage 3 (trypsin-EDTA 0.05%, Gibco) and seeded into collagen sponges (Symatèse BiomatĂ©riaux, Chapanost, France) at a density of 0.5 million BMMSCs/sponge.
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