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  • This medium was composed of Dulbecco's modified Eagle's medium with high glucose (DMEM-HG, Gibco) supplemented with 10% fetal bovine serum (FBS, Sigma), sodium pyruvate (110 μg/ml, Gibco), bFGF (1 ng/ml, Miltenyi Biotech), 1% penicillin-streptomycin (Gibco), chondrogenic supplements (PAD: proline (40 μg/ml, Sigma), -ascorbic acid-2-phosphate (50 μg/ml, Sigma) and dexamethasone (0.1 μM, Sigma).
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