PropertyValue
nif:beginIndex
  • 0 (xsd:integer)
nif:broaderContext
nif:endIndex
  • 508 (xsd:integer)
nif:isString
  • The absence of an enrichment step in HydraPsiSeq implies that a substantial coverage at all U positions in a given RNA sequence must be achieved (sequencing depth of 20-25 millions of reads for eukaryotic rRNA, see below), but, in return, HydraPsiSeq allows highly sensitive, reliable and precise quantification of ψ residues in RNA and, with inclusion of a calibration of the PsiScore signal to natural unmodified RNA or synthetic transcript, even to reach an absolute measurement of the modification level.
rdf:type