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  • The reliability of pseudouridine detection by HydraPsiSeq was evaluated by Receiver Operating Characteristic (ROC) curves and associated Matthews correlation coefficient (MCC) values for WT yeast rRNA containing 47 ψ residues altogether ( [60]), of which 46 are installed by Cbf5 as part of H/ACA snoRNPs ( [47]), while formation of ψ50 in 5S rRNA is catalyzed by the stand-alone enzyme Pus7 ( [61]).
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