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  • Inspection of cleavage efficiencies in yeast rRNA (used here as initial model and containing 47 known ψ sites, one each in 5S and 5.8S rRNAs, 14 in 18S rRNA and 31 in 25S rRNA ( [60])), demonstrated that, as expected, cleavage at A, C and G is relatively marginal (NormUcount < 5), while cleavage at U residues is very efficient (NormUcount ranging from Zero to 50-60), see Figure 1B (left panel) for 18S rRNA data, similar profiles were also obtained for 25S rRNA.
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