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  • Following anesthesia, tracheotomy was performed, and pigs were mechanically ventilated (Servo-i ventilator, Solna, Sweden) using volume-control mode at a Vt of 6 mL/kg, a respiratory rate of 30 breaths/min, FiO of 1.0, a inspiration-to-expiration time ratio (I:E) of 1:2, and PEEP of 5 cmHO.
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