Additionally, three TMA sections per patient were obtained and immunohistochemistry was performed with antibodies directed against PD-1(Clone NAT105; ready-to-use; 32 min; cat#760-4895), PD-L1(Clone NAT105; ready-to-use, 60 min; cat#790-4905) and PAN-cytokeratin (Clone A1/A3, 1:100; 16 min; Dako Agilent; cat#GA005361-2).
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