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  • For fluorescent detection, the first primary antibody was incubated followed by detection using a goat anti-mouse (GaM-HRP, 12 min; OmniMap anti-Ms HRP (RUO), DISCOVERY; cat#760-4310) or goat anti-rabbit (GaR-HRP; 16 min; OmniMap anti-Rs HRP (RUO), DISCOVERY; cat#760-4311) secondary antibody conjugated to horseradish peroxidase (HRP).
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