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  • To test the imaging properties of the microfluidic devices, 6 days post fertilization (dpf) zebrafish larvae expressing the genetically encoded Ca-indicator GCaMP6s under the control of the pan-neuronal elavl3 (formerly HuC) promoter [9] in the crystal background [10] were injected into the NeuroExaminer and oriented manually (Fig. 2a, e; Supplementary Movie [8]).
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