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  • Spheroid cultures were obtained by seeding 2.5 × 10 A431 cancer cells/well in a round bottom non-treated tissue culture 96 well-plate (Greiner Bio-one, Kremsmünster, Austria) in phenol red free RPMI-1640 medium (Sigma Chemical Co.) containing 10% fetal calf serum (FCS) and supplemented with 20% methyl cellulose stock solution. 3.6.3.
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