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Cell lines were maintained in the logarithmic phase at 37 °C in a 5% carbon dioxide atmosphere using RPMI-1640 or F-12 culture medium (Euroclone) containing 10% fetal calf serum (Euroclone, Milan, Italy), antibiotics (50 units per mL penicillin and 50 μg mL streptomycin), and 2 mM L-glutamine. 3.6.1.
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