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Fatty acid methyl esters (FAME) of freeze-dried Longissimus muscle were prepared (in duplicate) by direct bimethylation according to the method described by [16], and were analyzed using a gas chromatograph (Perkin-Elmer Autosystem-1:A, Waltham, MA, USA) equipped with a flame ionisation detector and an Omegawax 320 capillary column (30 m × 0.32 mm internal diameter; 0.25 mm film thickness) with polyethylene glycol as the stationary phase (Supelco, Bellefonte, PA, USA).
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