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  • After blocking with 5% skimmed milk at room temperature for 2 hrs, the membranes were incubated with rabbit anti-human CBX4 (1:1000; Abcam, Cambridge, UK), Cyclin A2 (1:1000; Abcam, Cambridge, UK), CDK2 (1:1000; Abcam, Cambridge, UK) or mouse anti-human β-actin (1:5000; Abcam, Cambridge, UK) monoclonal primary antibodies at 4°C overnight.
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