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  • Membranes were incubated with the following primary antibodies diluted in TBST containing 1% bovine serum albumin (BSA; ThermoFischer Scientific): EphA4 (1: 1000; overnight at 4 °C; Invitrogen, 37-1600), β-actin (1:5 000; 15 min at RT; Sigma, A5441), α-tubulin (1: 10 000; 10 min at RT; Sigma, T6199) and GAPDH (1:10 000; 1 h at RT; Ambion, AM4300).
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