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  • Briefly, tissue was homogenized in RIPA buffer (Sigma-Aldrich) supplemented with Complete EDTA-free Cocktail (Roche), phosSTOP (Roche) and PMSF (ThermoFischer Scientific), by means of mechanical disruption using Lysing Matrix D beads (MP Biomedicals) and a MagNa Lyser oscillator (Roche) at 6 500 rpm for 30 s thrice with 1 min interval on ice.
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