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  • Briefly, up to 1 µg of RNA was used to prepare cDNA with the SuperScript III First-Strand Synthesis System (ThermoFischer Scientific) and quantitative PCRs were performed with the StepOnePlus (Life Technologies), the TaqMan Fast Universal PCR Master Mix 2X (Life Technologies) and the following Taqman assays (IDT): Epha4 (Mm.PT.58.13545379), Polr2a (Mm.PT.58.13811327) and Gapdh (Mm.PT.39a.1).
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