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  • Whole cell lysates were prepared by adding per well of 9.6 cm, 300 μl of lysis buffer containing 1% IGPAL, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate (SDS), PBS without calcium without magnesium, 5 mM EDTA, 1 mM NaVO, 20 mM NAF, and 1 mM DL-dithiothreitol, and supplemented with a protease Inhibitor Cocktail (all reagents were obtained from Sigma, France).
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