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  • For adipocyte differentiation, ASC were amplified until 80% of confluency was reached and culture medium replaced by differentiation medium containing Dulbecco's modified Eagle medium/Nutrient Mixture F12 (DMEM/F12; Dominique Dutscher, France), FBS 10% (Hyclone, France), 100 U/ml penicillin, 100 μg/ml streptomycin, 2 mM glutamine, 1 μg/ml insulin, 0.5 μM dexamethasone, 2 nM Triiodo-L-Thyronine 3 (T3), 0.5 μM 3-isobuthyl-1-methylxanthine (IBMX), 2 μM rosiglitazone, and 10 μg/ml transferrin, all from Sigma-Aldrich.
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