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  • The pelleted stromal fractions were suspended in culture medium supplemented with 100 μM ascorbic acid (Sigma-Aldrich, France), 5 ng/ml human bFGF (eBioscience, France), and 5 U/ml heparin sodium (Sigma-Aldrich) and plated in 2 wells of 9.6 cm (Dutscher).
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