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  • At the end of the process, digested adipose tissues were filtered on a 100 μm nylon filter (Dutscher) and suspended in culture medium containing Dulbecco's modified Eagle's medium-high glucose (DMEM-HG) supplemented with 2 mM L-glutamine-L-alanyl (stable glutamine), 1 mM pyruvate, 100 U/ml penicillin, 100 μg/ml streptomycin, 25 mM hepes, and 10% fetal calf serum from South America (all culture medium components were from Dominique Dutscher, France).
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