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  • Pieces were incubated for 1 hour at 37°C in digestion buffer containing DPBS with calcium and magnesium supplemented with 2 mM glucose (Sigma-Aldrich, France), 1% bovine serum albumin (Dominique Dutscher), and 1 mg/ml collagenase (Sigma-Aldrich, France) and vortexed every 10 minutes.
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