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  • Metabolite concentrations were measured in supernatants of ASC placed for the indicated time in culture medium composed of DMEM without glucose, without pyruvate, without glutamine, and without red phenol (PAN-Biotech, France) supplemented with 25 mM glucose, antibiotics, 20% FCS, 25 mM hepes, nonessential amino acid 1x (PAA, France), 4 mM L-glutamine (Corning, France), 1 mM pyruvate (Dutscher, France), 100 μM ascorbic acid, 5 ng/ml human bFGF, and 5 U/ml heparin sodium.
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