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  • To quantify the percentage of codons that are invariant and experiencing extreme purifying selection, we counted the number of codons in each of the original four alignments (whole gene, motor domain, neck, and CBD) that had dN and dS values of zero and divided this by the total number of codons in the alignment to determine the percentage of codons that are invariant and experiencing extreme purifying selection (tables 1Teleost myo5 dN/dSValues and [2]Percentage of Invariant Codons in Teleost myo5 Genes ).
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