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  • Sperm concentration and motility were assayed using a computer-aided sperm analyses (CASA) system coupled to a phase contrast microscope (Nikon Eclipse model 50i; Nikon Instruments Europe B.V., Izasa S.A.; negative contrast) and employing Sperm Class Analyzer (SCA, Barcelona, Spain) v.4.0. software (Microptic S.L., Barcelona, Spain) [35].
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