nif:isString
|
-
We utilized intron probes for OR RNA FISH for three reasons: (i) we can design longer intron than exon probes (enhanced sensitivity); (ii) for genes (like ORs) expressed at low levels, unprocessed RNAs at the native locus are more spatially concentrated than processed RNAs in the cytoplasm (enhanced sensitivity); (iii) the one-spot (monoallelic) nuclear signal is an important validation of an OR signal (enhanced specificity).
|