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  • Prior to each measurement, the VO2 and VCO2 analyzers were calibrated using a known mixture of gases (16.00 ± 0.04% O2 and 5.00 ± 0.1% CO2, Riessner-Gase GmbH & Co., Lichtenfels, Germany) and the expiratory flow meter calibrated with a 3-L syringe (Hans Rudolph Inc., Kansas City, MO).
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