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The thermal cycling conditions using a viiA™7 apparatus (Applied Biosystems) for Experiment 1 and StepOnePlus™ (Applied Biosystems) for Experiment 2 and 3 were 10 min at 95°C, followed by 40 cycles consisting of a denaturing stage at 95°C for 15 s and as annealing/extension stage at 60°C for 1 min.
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