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  • PhysiCell—physics-based multicellular simulator—is an open source agent-based simulator that provides both the stage and the players for studying many interacting cells in dynamic tissue microenvironments. We demonstrate PhysiCell by simulating the impact of necrotic core biomechanics, 3-D geometry, and stochasticity on the dynamics of hanging drop tumor spheroids and ductal carcinoma in situ (DCIS) of the breast. The PhysiCell source code, examples, documentation, and support are available under the BSD license at http://PhysiCell.MathCancer.org and http://PhysiCell.sf.net. PhysiCell is written in cross-platform compatible C++ and is self-contained (with minimal dependencies). We have tested PhysiCell on Windows through MinGW-w64, and on OSX and Linux via g++. Downloading PhysiCell: Users can download a PhysiCell release at: You can get the most recent virtual appliance by browsing to: https://sourceforge.net/projects/physicell/files/PhysiCell/. Then browsing a recent release directory (e.g., PhysiCell 1.2.2), and downloading the ova file. https://github.com/MathCancer/PhysiCell/releases/latest PhysiCell is licensed under the (3-clause) BSD license, which is compatible with commercial products and can be included in GPL-licensed projects.
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