PCR fragments of 271 bp and 181 bp containing sequence from -124 to +147 of phzX were amplified from genomic DNA of P. chlororaphis 30–84 and pUC57-Enh using the primers, phzXF1/phzXR1 (S1 Table) and cloned into the promoter trap vector pGT2-lacZ resulting plasmids pJMYX1 and pJMYX2, respectively (Fig 2 and Table 1).
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