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  • Construction of phzX transcriptional fusion derivatives: To determine the function of the 5’-UTR of phenazine biosynthetic operon in phenazine gene expression, a 1.5-Kb DNA fragment contains a deletion of 90-bp in the 5’-UTR of phenazine biosynthetic operon was synthesized (GeneScript, Piscataway, NJ) and obtained as pUC57-Enh (Table 1).
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