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Samples were submitted (delivered or mailed) to IDEXX Laboratories (Sacramento, California) for real-time quantitative polymerase chain reaction (RT-PCR) testing (test code 2512 RealPCR™ FURD panel) designed to detect the following feline upper respiratory disease agents: Chlamydophila felis, feline calicivirus (FCV), feline herpesvirus-1 (FHV-1), Bordetella bronchiseptica and Mycoplasma felis.
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