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  • Briefly, 2.5 μL of the sample lysates, 900nM of each forward and reverse primer, 300 nM of the probe, 12.5 μL TaqMan®2x Universal PCR MasterMix (Applied Biosystems, Warrington, UK) and an exogenous internal positive control (IPC; Applied Biosystems, Warrington, UK) were introduced in each reaction well and nuclease-free water up to a total volume of 25 μL.
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