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Based on this time course of infection, the experimental period was divided into 4 phases for analysis (Fig 1): (1) pre-infection (days 1-11), when animals were free of infection; (2) infection (days 12-15), after animals were inoculated with ASF virus but with no ASF virus detected; (3) qPCR detection (days 16-18), during which ASF virus was qPCR-detected in at least one animal; and (4) clinical detection (days 19-23), when clinical signs of ASF were evident in at least one animal, until the end of the experiment.
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