For microscopic assessment of viability, PCLS were incubated with 2 μM Calcein AM and 5 μM Ethidium homodimer-I (EthD-1) for 45 min at room temperature in the dark on an orbital shaker (150 rpm) using the Live/Dead® Viability/Cytotoxicity kit from Life Technologies (Darmstadt, Germany), as previously described [7].
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