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Bicinchoninic acid (BCA) total protein kit, Earle’s Balanced Salt Solution (EBSS) and Dulbecco’s Modified Eagle’s Medium Nutrient Mixture F-12 Ham (DMEM, pH 7.2-7.4, without phenol red) with L-glutamine and 15 mM HEPES were obtained from GIBCO (Thermo Fisher Scientific, Rockford, IL, USA).
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