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  • Specific primers for CXCL1 were designed using Oligo Calc (Kibbe, 2007) as follow: 5’- CACTGCACCCAAACCGAAGTCATA-3’; 5’-TCTCCGTTACTTGGGGACACCTTT -3’; A DNA Master Plus SYBR Green Kit (Roche Diagnostics, France) was used in a LightCycler 2.0 (Roche Diagnostics, France) with an initial incubation of 10 min at 95°C, 40 amplification cycles of ten seconds at 95°C, of 8 sec at 60°C, and of 10 sec at 72°C, during which the fluorescence data were collected.
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