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  • Real-time PCR was performed using the DNA Master Plus SYBR Green Kit (Roche Diagnostics, France) in a LightCycler 2.0 (Roche Diagnostics, France) with an initial incubation of 10 min at 95°C, 40 amplification cycles of ten seconds at 95°C, of eight seconds at 60°C, and of ten seconds at 72°C, during which the fluorescence data were collected.
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