Real-time PCR gene-specific primers for s100a8, s100a9 and β-actin were designed using Oligo Calc (Kibbe, 2007) as follow: s100a8, 5’-ACCATGCCCTCTACAAGAA TGACT-3’; 5’-ACTCCTTGTGGCTGTCTTTGTG-3’; s100a9, 5’-AACCAGGACAATCAG CTGAGCTTT-3’; 5’-AGGCCATTGAGTAAGCCATTCCC-3’; β-actin, 5’-ACCACAGCTGAGAGGGAAATCGT-3’; 5’-AACCGCTCGTTGCCAATAGTGA-3’.