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Proportions of the different leukocyte populations were determined by flow cytometry using the following rat anti-mouse antibodies: anti- F4/80-APC (dilution 1:200; eBioscience, clone BM8), anti-SiglecF-PE (dilution 1:200, BD Bioscience, clone E50-2440) and Ly6G-FITC (dilution 1:200, BD Bioscience, clone 1A8).
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