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  • Analysis of cytokines and S100A9 in pleural and broncho-alveolar lavage fluids: Pleural and bronchoalveolar lavage (BAL) fluids were analyzed by ELISA for the content of IFN-γ, TNF-α, IL-10, IL-4, IL-1β, IL-6 and IL-17 ELISA kit (eBiosciences SAS, France), MCP-1 and CXCL1 ELISA kit (Peprotech, France), S100A9 and IL-33 (R&D, UK) following manufacturers’ guidelines.
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