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A two-step sample collection was applied: 1) the test set (collection between 2011.06.30.- 2012.04.13.) contained 29 OSCC, 25 age-matched and 8 young controls for method development and biomarker identifications and 2) a reference set (collection between 2013.05.09–2016.02.29) containing 26 OSCC, 12 age-matched and 7 young controls for biomarker verification.
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