Dinaciclib (25 nM), a clinically relevant dose [19], or vehicle was added and incubated for 24 h, after which adherent cells were trypsinized, washed with PBS, fixed with cold 70% ethanol and incubated with RNase A (100 μg/mL; Sigma) and propidium iodide (PI, 5 μg/mL; Sigma) at 37°C for 15 min.
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