The murine oral cancer cell line AT-84, originating from C3H mice [30], was generously provided by Dr. Shulin Li at MD Anderson (Houston, TX, USA) and grown in RPMI-1640 with 10% FBS, 2 mM L-glutamine, 0.1 mM non-essential amino acids,10 mM N-2-HEPES buffer, 100 units penicillin/ml, and 100 μg streptomycin/ml at 37°C with 5% CO2.
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