In brief, Fc receptors were blocked with anti-CD16/CD32 antibody (eBioscience, San Diego, CA, USA) and then cells were incubated with the appropriate conjugated antibodies (CD11b [M1/70], Ly6C [AL-21], Ly6G [1A8], Il-4Rα [mIL4R-M1],BD Biosciences, San Jose, CA, USA) for 1 h at 4°C.
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