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  • All mice were anesthetized again (100 mg/kg ketamine mixed with 10 mg/kg xylazine, i.p. ), the dorsal skin was shaved, cleaned with alcohol, and wounded by placing two full-thickness excisional wounds through the dorsal skin using 3.5 mm sterile biopsy punches (Miltex Instrument Company, Plainsboro, NJ, USA) just below the shoulder blades to prevent mice from licking their own wounds.
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