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Phagocytosis and Fluid Phase Uptake: Phagocytosis and fluid phase uptake were determined as described previously [5] by incubating cells for 20 min in suspension in HL5 medium containing either 1-μm-diameter Fluoresbrite YG carboxylate microspheres (Polysciences, Warrington, PA) or Alexa647-dextran (Life Technologies, Eugene, OR).
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