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The solvent was removed under nitrogen and the extract re-suspended in methanol for quantitative determination using Hydrophilic interaction liquid chromatography (HILIC) on a Thermo perfluorinated phenyl (PFP) column using LC-MS/MS with multiple reaction monitoring (MRM) on an Agilent 1100 LC system interfaced to an ABSciex API 4000 tandem mass spectrometer.
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