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Under anesthesia, the head was shaved, eye lubricant was applied, the analgesic buprenorphine (0.02 mg/kg) was administered subcutaneously under the abdominal skin, and a mixture of lidocaine (7 mg/kg) and bupivacaine (3.5 mg/kg) was injected subcutaneously over the skull incision site and area in contact with the stereotaxic ear bars.
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