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In this analysis, values were log-transformed to conform to assumptions of linearity, and linear mixed effects models (NLME package version 3.1–118 in R) were used to account for ACTH challenge (I and II) and individual identity as random factors, because two individuals were tested in both challenges (F1 and M4) and because we had multiple samples per individual which were not evenly distributed among the three day time periods.
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